Using SNP markers to assess genetic variability of Plasmodiophora brassicae in Canada
Dr. Bruce Gossen
Dr. Mary Ruth McDonald, Dr. Stephen Strelkov, Dr. Gary Peng, Fengqun Yu
SaskCanola, Manitoba Canola Growers
April 2015 - March 2018
Clubroot cause by P. brassicae had only been reported in Quebec three times before its discovery in Alberta on canola in 2003. The source of the clubroot outbreak on canola has never been determined – in addition to its genetic variability.
Using SNP markers, assess genetic relatedness of initial pathogenic P. brassicae lines on canola across the Canadian prairies. Analysis of genotypic similarity will be used to indicate the origin of the initial sources of inoculum, to determine if the outbreak was the result of one or multiple introductions, and to determine if the new pathotypes(s) that have been reported in Alberta (ie: 5X) are genetically similar to each other and the predominant pathotypes.
A DNA extraction protocol for P. brassicae was developed which is suitable for whole-genome sequencing.
Gaining a better understanding of Plasmodiophora brassicae at the genetic level will help understand the durability of resistance, as well as provide breeders with resources to determine optimal clubroot resistance genetics.
Keywords:SNP markers, Clubroot