A rapid molecular assay to identify Plasmodiophora brassicae pathotypes from plant and soil samples

The Challenge

New Plasmodiophora brassicae pathotypes can overcome host resistance are emerging and there is currently no quick and easy way of identifying them.

The Project

To generate an effective, sensitive, and rapid molecular assay (PCR-based) to identify abundance and diversity of P. brassicae pathotypes in soil and plant samples; and to use the genetic variability among pathotypes to establish their relationships and genes of interest related to infection.

The Results

This project developed two molecular tests: an RNase H2-dependent PCR (rhPCR) assay, and a SNaPshot assay, both of which could clearly distinguish between pathotype clusters in a collection of 38 single-spore isolates of P. brassicae.

Grower Benefits

This molecular assay would help monitor the diversity and abundance of pathotypes found, ultimately supporting farmers in choosing cultivar selection and the development of canola with novel sources of resistance.